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KMID : 0616120160470010079
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Korean Journal of Pharmacognosy
2016 Volume.47 No. 1 p.79 ~ p.83
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Simultaneous Determination of Isoegomaketone and Perillaketone in Perilla frutescens (L.) Britton Leaves by HPLC-DAD
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Nam Bo-Mi
Lee Seung-Young
Kim Jin-Baek
Kang Si-Young
Jin Chang-Hyun
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Abstract
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This study developed an HPLC analysis method for the determination of isoegomaketone (IK) and perillaketone (PK) in Perilla frutescens (L.) Britton leaves. P. frutescens ethanol extract was optimized through an HPLC analysis using a C18 column (250 X 4.6 mml, D, S-5 μm, 12 nm) with gradient elution of water and acetonitrile as the mobile phase at a flow rate of 1 mL/min and a UV detection wavelength of 254 nm. The results of this method showed linearity in the calibration curve
at a coefficient of correlation (R 2 ) of IK 0.9995, PK 0.9998. The limits of detection (LOD) for IK and PK were 0.234 μg/mL
and 0.952 μg/mL. The limits of quantification (LOQ) for IK and PK were 0.017 μg/mL and 0.043 μg/mL. The inter-day precision RSDs of IK and PK in the P. frutescens were 1.25 to 2.69% and 0.36 to 1.10%, respectively, and the intra-day precision RSDs of IK and PK were 0.96 to 2.51% and 0.90 to 1.93%, respectively. The accuracies of IK and PK were 96.31 to 97.92% and 101.26 to 105.14%. In conclusion, this method was applied successfully to the detection of IK and PK in P. frutescens.
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KEYWORD
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Perilla frutescens (L.) Britton , Isoegomaketone , Perillaketone , HPLC-DAD
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